In the first week of this course we will begin to acquire some of the basic skills
needed to examine several components of the inflammatory cascade, a series of
responses that are integrally intertwined with the immune system. First we will begin
to acquire some basic skills in tissue culture, and the purification of selected cells
associated with the immunoinflammatory system (i.e., peripheral blood [PBL]
monocytes and neutrophils [PMN or polymorphonuclear cells]). Then we will learn
how to identify them using morphologic criteria, and how to assess a couple of
functions associated with monocytes/macrophages - phagocytosis (a subfunction of
antibody-dependent cellular cytotoxicity) and activation-dependent monokine
production.
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